Fluorescence lifetime imaging

Photograph of the current confocal microscope.
Photograph of the current confocal microscope.

We have built a confocal laser scanning microscope to perform fluorescence lifetime imaging to work on tissue diagnostics. The setup uses time-correlated single photon counting (TCSPC), together with a tunable picosecond pulsed excitation source.

Ongoing investigations include a collaboration with the Department of Chemistry and Biochemistry at the University of Bern, whereby the interactions of photosensitizers with their micro-environments are studied in the frame of photodynamic therapy.